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RBC - Performance of combinatorial peptide libraries in capturing the low-abundance proteome of red blood cells

ABSTRACT: GeLC-MS/MS analysis of human RBCs

INSTRUMENT(S): LTQ-Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Erythrocyte

SUBMITTER: Emanuele Alpi

LAB HEAD: Emanuele Alpi

PROVIDER: PRD000154 | Pride | 2009-12-01

REPOSITORIES: Pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_10042.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_10042.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_10042.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_10043.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_10043.pride.mztab.gz	Mztab

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Publications

Performance of combinatorial peptide libraries in capturing the low-abundance proteome of red blood cells. 2. Behavior of resins containing individual amino acids.

Bachi Angela A Simó Carolina C Restuccia Umberto U Guerrier Luc L Fortis Frederic F Boschetti Egisto E Masseroli Marco M Righetti Pier Giorgio PG

Analytical chemistry 20080415 10

<h4>Unlabelled</h4>Sixteen different amino acids (Arg, Asn, Asp, Gln, Glu, Gly, His, Ile, Lys, Phe, Pro, Ser, Thr, Trp, Tyr, Val) have been separately linked to chromatographic beads and used for studying the mechanism of binding of such baits to proteins, as represented by the cytoplasmic proteome of the human red blood cell (RBC). The 16 different amino acid columns were confronted with equal amounts of RBC lysate, washed to remove unbound material, and eluted with denaturing agents. All eluat ...[more]

PMID: 18410134

Publication: 1/2

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