Identification of ciliary neurotrophic factor receptor alpha as a mediator of neurotoxicity induced by extracellular alpha-synuclein

ABSTRACT: MES cells were treated with vehicle or 250 nM extracellular alpha-synuclein and plasma membrane proteins were labeled with biotin, followed by affinity purification. The biotinylated enriched membranous proteins were incubated with an alpha-synuclein peptide array membrane, washed extensively with TBS-T, followed by incubation with avidin-HRP. The positive spots were excised and the bound proteins were eluted. The elutions from consecutive spots were combined before trypsin digestion and analysis of peptides by LC-MS/MS.

INSTRUMENT(S): Thermo Finnigan LCQ Deca XP

ORGANISM(S): Mus Musculus X Rattus Norvegicus

TISSUE(S): Dopaminergic Neuron, Permanent Cell Line Cell

SUBMITTER: Jing Zhang  

LAB HEAD: Jing Zhang

PROVIDER: PRD000155 | Pride | 2009-12-01

REPOSITORIES: Pride