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Identification of ciliary neurotrophic factor receptor alpha as a mediator of neurotoxicity induced by extracellular alpha-synuclein

ABSTRACT: MES cells were treated with vehicle or 250 nM extracellular alpha-synuclein and plasma membrane proteins were labeled with biotin, followed by affinity purification. The biotinylated enriched membranous proteins were incubated with an alpha-synuclein peptide array membrane, washed extensively with TBS-T, followed by incubation with avidin-HRP. The positive spots were excised and the bound proteins were eluted. The elutions from consecutive spots were combined before trypsin digestion and analysis of peptides by LC-MS/MS.

INSTRUMENT(S): LCQ Deca, instrument model

ORGANISM(S): Mus Musculus X Rattus Norvegicus

TISSUE(S): Dopaminergic Neuron, Permanent Cell Line Cell

SUBMITTER: Jing Zhang

PROVIDER: PRD000155 | Pride | 2009-12-01

REPOSITORIES: Pride

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α-Synuclein promotes H4R3me2s through interacting with BAF-PRMT5 complex

Project description:We conducted a screening for nuclear proteins which interact with α-synuclein by mass-spectrometry. As a result, we found that α-synuclein interact with BAF complex. Furthermore, overexpressed α-synuclein increased global H4R3me2s by promoting interaction between BAF complex and PRMT5 in cultered cells. In this study, we explored the influence of overexpressed α-synuclein on H4R3me2s by ChIP-seq using anti-H4R3me2s antibody.

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Dietary intake of α-ketoglutarate ameliorates α-synuclein pathology in a Parkinson’s disease mouse model

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2022-10-02 | GSE214446 | GEO

Phenotypic manifestation of alpha-synuclein strains from Parkinson’s disease and multiple system atrophy in human dopaminergic neruons

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Expression data from oligodendroglial cell line

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2013-10-28 | E-GEOD-51781 | biostudies-arrayexpress

Expression data from oligodendroglial cell line

2013-10-28 | GSE51781 | GEO

Alpha-synuclein deficiency affects brain Foxp1 expression and ultrasonic vocalization

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Hsp110 Mitigates α-Synuclein Pathology In Vivo

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