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Tsiotis - Unraveling persistent host cell infection with Coxiella burnetii by quantitative proteomics

ABSTRACT: Vero cells infected with Coxiella NM vs NM persistent

INSTRUMENT(S): Agilent XCT, LTQ-Orbitrap

ORGANISM(S): Cercopithecus Aethiops (green Monkey) (grivet) Chlorobium Tepidum

DISEASE(S): Q Fever

SUBMITTER: Pieter-Jan De Bock

LAB HEAD: Pieter-Jan De Bock

PROVIDER: PRD000263 | Pride | 2011-10-12

REPOSITORIES: Pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_13082.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_13082.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_13082.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_17115.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_17115.pride.mztab.gz	Mztab

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Publications

Unraveling persistent host cell infection with Coxiella burnetii by quantitative proteomics.

Vranakis Iosif I De Bock Pieter-Jan PJ Papadioti Anastasia A Samoilis Georgios G Tselentis Yannis Y Gevaert Kris K Tsiotis Georgios G Psaroulaki Anna A

Journal of proteome research 20110815 9

The interaction between the immune system and invading bacteria is sufficient to eradicate microorganisms for the majority of bacterial infections, but suppression of the microbicidal response leads to reactivation or chronic evolution of infections and to bacterial persistence. To identify the cellular pathways affected by bacterial persistence, we applied the MS-driven combined fractional diagonal chromatography (COFRADIC) proteomics technique for a comparative study of protein expression in t ...[more]

PMID: 21790200

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Host cell-free growth of the Q fever bacterium Coxiella burnetii

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Host cell-free growth of the Q fever bacterium Coxiella burnetii

Project description:The inability to propagate obligate intracellular pathogens under axenic (host cell-free) culture conditions imposes severe experimental constraints that have negatively impacted progress in understanding pathogen virulence and disease mechanisms. Coxiella burnetii, the causative agent of human Q (Query) fever, is an obligate intracellular bacterial pathogen that replicates exclusively in an acidified, lysosome-like vacuole. To define conditions that support C. burnetii growth, we systematically evaluated the organism’s metabolic requirements using expression microarrays, genomic reconstruction, and metabolite typing. This led to development of a complex nutrient medium that supported substantial growth (~ 3 log10) of C. burnetii in a 2.5% oxygen environment. Importantly, axenically grown C. burnetii were highly infectious for Vero cells and exhibited developmental forms characteristic of in vivo grown organisms. Axenic cultivation of C. burnetii will facilitate studies of the organism’s pathogenesis and genetics, and aid development of Q fever preventatives such as an effective subunit vaccine. Furthermore, the systematic approach used here may be broadly applicable to development of axenic media that support growth of other medically important obligate intracellular pathogens.

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Transcriptional profiling of Coxiella burnetii reveals extensive cell wall remodeling in the small cell variant developmental form

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