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Combine Approach HNE - Combined proteomic approaches for the identification of specific amino acid residues modified by 4-hydroxy-2-nonenal under physiological conditions

ABSTRACT: HNE modifications

INSTRUMENT(S): LTQ

SUBMITTER: MÂªLuisa hernÃ¡ez

LAB HEAD: MÂªLuisa hernÃ¡ez

PROVIDER: PRD000286 | Pride | 2010-10-21

REPOSITORIES: Pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_13513.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_13513.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_13514.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_13514.xml.gz	Xml

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Publications

Combined proteomic approaches for the identification of specific amino acid residues modified by 4-hydroxy-2-nonenal under physiological conditions.

Mendez Dario D Hernaez Maria Luisa ML Diez Amalia A Puyet Antonio A Bautista Jose M JM

Journal of proteome research 20101008 11

Proteins modified by 4-hydroxy-2-nonenal (HNE) are cellular markers of oxidative stress in health and disease. HNE is generated by free radical chain reactions during oxidative stress as a major end-product of the oxidative fatty acid metabolism. Identification and quantitative analysis of HNE-modified proteins are readily performed by using specific antibodies raised against them. Further on, the identification of the amino acid residues involved in the HNE-modification is an additional step in ...[more]

PMID: 20818828

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