Dataset Information

Membrane-SPINE - Membrane-SPINE: an improved method to identify protein-protein interaction partners of membrane proteins in vivo

ABSTRACT: CpxA with Formaldehyde

INSTRUMENT(S): LTQ-FT Finnigan

ORGANISM(S): Salmonella Enterica Subsp. Enterica Serovar Typhimurium Str. Sl1344

SUBMITTER: Volker Steffen Mueller

LAB HEAD: Volker Steffen Mueller

PROVIDER: PRD000292 | Pride | 2011-08-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_13634.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_13634.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_13635.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_13635.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_13636.pride.mgf.gz	Mgf

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Publications

Membrane-SPINE: an improved method to identify protein-protein interaction partners of membrane proteins in vivo.

Müller Volker S VS Jungblut Peter R PR Meyer Thomas F TF Hunke Sabine S

Proteomics 20110407 10

Membrane proteins are crucial for many essential cellular processes. As membrane proteins function in complexes, methods to detect and to characterize membrane protein-protein interactions are undoubtedly required. Therefore, we developed the "Membrane-Strep-tagged protein interaction experiment" (Membrane-SPINE) that combines the specific purification of a Strep-tagged membrane protein with the reversible fixation of protein complexes by formaldehyde cross-linking. In combination with MS analys ...[more]

PMID: 21472855

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