Proteomics

Dataset Information

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A_niger_microsome - Proteomic analysis of the secretory response of Aspergillus niger to D-maltose and D-xylose


ABSTRACT: Shotgun proteomics of microsomal enriched fraction 2 h after addition 0f 10mM xylose to sorbitol grown mycelium

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Aspergillus Niger (strain Cbs 513.88 / Fgsc A1513)

SUBMITTER: Peter Schaap  

PROVIDER: PRD000334 | Pride | 2012-05-17

REPOSITORIES: Pride

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Publications

Proteomic analysis of the secretory response of Aspergillus niger to D-maltose and D-xylose.

de Oliveira José Miguel P Ferreira JM   van Passel Mark W J MW   Schaap Peter J PJ   de Graaff Leo H LH  

PloS one 20110617 6


Fungi utilize polysaccharide substrates through extracellular digestion catalyzed by secreted enzymes. Thus far, protein secretion by the filamentous fungus Aspergillus niger has mainly been studied at the level of individual proteins and by genome and transcriptome analyses. To extend these studies, a complementary proteomics approach was applied with the aim to investigate the changes in secretome and microsomal protein composition resulting from a shift to a high level secretion condition. Du  ...[more]

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