Proteomics

Dataset Information

0

Blumeria graminis f.sp. hordei DH14 proteogenomics 2010


ABSTRACT: ACN_BlumORF_20101027_F009873 In solution tryptic digest of ACN fractions and crude extracts of sporulating hyphae nRPLC 130 min 2-32%ACN and 20min 32-48%ACN gradients online with nESI LTQ OrbitrapXL MSMS (CID) db: Blumeria ORF db (contigs) Mascot search 99% confidence

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Blumeria Graminis F. Sp. Hordei Dh14

TISSUE(S): Hyphal Cell, Hypha, Conidium, Haustorium

SUBMITTER: Laurence v. Bindschedler  

PROVIDER: PRD000366 | Pride | 2013-07-09

REPOSITORIES: Pride

altmetric image

Publications

Proteogenomics and in silico structural and functional annotation of the barley powdery mildew Blumeria graminis f. sp. hordei.

Bindschedler Laurence V LV   McGuffin Liam J LJ   Burgis Timothy A TA   Spanu Pietro D PD   Cramer Rainer R  

Methods (San Diego, Calif.) 20110329 4


Blumeria graminis is an economically important obligate plant-pathogenic fungus, whose entire genome was recently sequenced and manually annotated using ab initio in silico predictions (Spanu et al. 2010, Science 330, 1543-1546). Employing large scale proteogenomic analysis we are now able to verify independently the existence of proteins predicted by ∼24% of open reading frame models. We compared the haustoria and sporulating hyphae proteomes and identified 71 proteins exclusively in haustoria,  ...[more]

Similar Datasets

2012-12-06 | PRD000730 | Pride
2016-10-04 | GSE68963 | GEO
2011-03-11 | GSE20279 | GEO
2017-03-27 | GSE69215 | GEO
2021-03-30 | GSE165730 | GEO
2012-06-11 | E-MTAB-682 | biostudies-arrayexpress
2009-06-10 | E-IPKG-7 | biostudies-arrayexpress
2009-06-10 | E-IPKG-8 | biostudies-arrayexpress
| PRJNA186718 | ENA
| PRJNA186717 | ENA