Colon cancer - Characterization of changes in the proteome in different regions of 3D multicell tumor spheroids

ABSTRACT: Proteins were extracted from different regions of HT29 colon carcinoma cells grown as multicell tumour spheroids. Changes in protein expression across the regions were determined by trypsin digestion iTRAQ 4-plex labelling, 2D separation using OffGel (24 fractions) and RP nanoHPLC, MALDI TOF-TOF MS/MS analysis and Mascot database searching.

INSTRUMENT(S): Bruker Ultraflex

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colonic Cancer Cell Line, Colon

SUBMITTER: Chris Sutton  

LAB HEAD: Chris Sutton

PROVIDER: PRD000369 | Pride | 2014-04-08

REPOSITORIES: Pride