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Fusarium graminearum WT phosphoproteome, non-limiting conditions

ABSTRACT: High throughput phosphoproteomics analysis of F. graminearum DOAM233423

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Fusarium Sp.

SUBMITTER: Christof Rampitsch

PROVIDER: PRD000382 | Pride | 2013-06-19

REPOSITORIES: Pride

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Phosphoproteome profile of Fusarium graminearum grown in vitro under nonlimiting conditions.

Rampitsch Christof C Tinker Nicholas A NA Subramaniam Rajagopal R Barkow-Oesterreicher Simon S Laczko Endre E

Proteomics 20120401 7

This study presents a high-throughput proteomic analysis of phosphopeptides from Fusarium graminearum strain DAOM 233423 grown in vitro without nutritional limitation. Using a combination of strong cation exchange (SCX) and immobilized metal affinity chromatography (IMAC) followed by LC-MS, we identified 2902 putative phosphopeptides with homologous matches to 1496 different proteins. Functional classification of the annotated protein set revealed that phosphopeptides from nuclear proteins with ...[more]

PMID: 22522806

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