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Methionine sulfoxide - Redox proteomics of protein-bound methionine oxidation

ABSTRACT: This project contains the COFRADIC data of two experimental set-ups (swapped labeling) in which methionine sulfoxides were identified in Jurkat cells stressed with hydrogen peroxide. We used a SILAC set-up in which cells were labeled with 12C5 methionine or with 13C5 methionine. One of the set-ups was treated with hydrogen peroxide, the other with H2O. Following lysis proteins were mixed in a 1/1 ratio. We then performed our methionine sulfoxide validation protocol onto the sample identifying methionine sulfoxides in the stressed Jurkat cells.

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: Bart Ghesquiere

PROVIDER: PRD000395 | Pride | 2011-03-18

REPOSITORIES: Pride

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Redox proteomics of protein-bound methionine oxidation.

Ghesquière Bart B Jonckheere Veronique V Colaert Niklaas N Van Durme Joost J Timmerman Evy E Goethals Marc M Schymkowitz Joost J Rousseau Frederic F Vandekerckhove Joël J Gevaert Kris K

Molecular & cellular proteomics : MCP 20110315 5

We here present a new method to measure the degree of protein-bound methionine sulfoxide formation at a proteome-wide scale. In human Jurkat cells that were stressed with hydrogen peroxide, over 2000 oxidation-sensitive methionines in more than 1600 different proteins were mapped and their extent of oxidation was quantified. Meta-analysis of the sequences surrounding the oxidized methionine residues revealed a high preference for neighboring polar residues. Using synthetic methionine sulfoxide c ...[more]

PMID: 21406390

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