Methionine sulfoxide - Redox proteomics of protein-bound methionine oxidation

ABSTRACT: This project contains the COFRADIC data of two experimental set-ups (swapped labeling) in which methionine sulfoxides were identified in Jurkat cells stressed with hydrogen peroxide. We used a SILAC set-up in which cells were labeled with 12C5 methionine or with 13C5 methionine. One of the set-ups was treated with hydrogen peroxide, the other with H2O. Following lysis proteins were mixed in a 1/1 ratio. We then performed our methionine sulfoxide validation protocol onto the sample identifying methionine sulfoxides in the stressed Jurkat cells.

INSTRUMENT(S): LTQ - Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: Bart Ghesquiere  

LAB HEAD: Bart Ghesquiere

PROVIDER: PRD000395 | Pride | 2011-03-18

REPOSITORIES: Pride