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Genetic analysis of MA4079 - Genetic analysis of MA4079, an aldehyde dehydrogenase homolog, in Methanosarcina acetivorans

ABSTRACT: Peptide mass fingerprinting of Methanosarcina acetivorans wt and mu3 differentially regulated bands obtained by submitting whole cell lysates to SDS-PAGE

INSTRUMENT(S): Bruker Daltonics micrOTOF series, instrument model

ORGANISM(S): Methanosarcina Acetivorans

SUBMITTER: Julian Langer

PROVIDER: PRD000398 | Pride | 2011-07-13

REPOSITORIES: Pride

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Genetic analysis of MA4079, an aldehyde dehydrogenase homolog, in Methanosarcina acetivorans.

Kliefoth Michael M Langer Julian D JD Matschiavelli Nicole N Oelgeschläger Ellen E Rother Michael M

Archives of microbiology 20110707 2

When Methanosarcina acetivorans grows on carbon monoxide (CO), it synthesizes high levels of a protein, MA4079, homologous to aldehyde dehydrogenases. To investigate the role of MA4079 in M. acetivorans, mutants lacking the encoding gene were generated and phenotypically analyzed. Loss of MA4079 had no effect on methylotrophic growth but led to complete abrogation of methylotrophic growth in the presence of even small amounts of CO, which indicated the mutant's inability ...[more]

PMID: 21735228

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