Proteomics

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Arabidopsis Golgi proteome - Isolation and proteomic characterization of the Arabidopsis Golgi defines functional and novel components involved in plant cell wall biosynthesis


ABSTRACT: The Golgi apparatus from Arabidopsis cell culture (Ler) were enriched by sucrose density centrifugation and purified by Free Flow Electrophoresis. Data from 2 fractions.

INSTRUMENT(S): QSTAR Elite

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Joshua Heazlewood  

LAB HEAD: Joshua Heazlewood

PROVIDER: PRD000424 | Pride | 2013-07-08

REPOSITORIES: Pride

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The plant Golgi plays a pivotal role in the biosynthesis of cell wall matrix polysaccharides, protein glycosylation, and vesicle trafficking. Golgi-localized proteins have become prospective targets for reengineering cell wall biosynthetic pathways for the efficient production of biofuels from plant cell walls. However, proteomic characterization of the Golgi has so far been limited, owing to the technical challenges inherent in Golgi purification. In this study, a combination of density centrif  ...[more]

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