Dataset Information

Improving reproducibility and sensitivity in identifying human proteins by shotgun proteomics

ABSTRACT: Not available

INSTRUMENT(S): ESI-ION-TRAP

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Attila Csordas

LAB HEAD: Attila Csordas

PROVIDER: PRD000465 | Pride | 2012-07-26

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_17917.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_17917.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_17917.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_17918.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_17918.pride.mztab.gz	Mztab

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Publications

Improving reproducibility and sensitivity in identifying human proteins by shotgun proteomics.

Resing Katheryn A KA Meyer-Arendt Karen K Mendoza Alex M AM Aveline-Wolf Lauren D LD Jonscher Karen R KR Pierce Kevin G KG Old William M WM Cheung Hiu T HT Russell Steven S Wattawa Joy L JL Goehle Geoff R GR Knight Robin D RD Ahn Natalie G NG

Analytical chemistry 20040701 13

Identifying proteins in cell extracts by shotgun proteomics involves digesting the proteins, sequencing the resulting peptides by data-dependent mass spectrometry (MS/MS), and searching protein databases to identify the proteins from which the peptides are derived. Manual analysis and direct spectral comparison reveal that scores from two commonly used search programs (Sequest and Mascot) validate less than half of potentially identifiable MS/MS spectra (class positive) from shotgun analyses of ...[more]

PMID: 15228325

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