Dataset Information
Improving reproducibility and sensitivity in identifying human proteins by shotgun proteomics
Ontology highlight
ABSTRACT:
INSTRUMENT(S): instrument model
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: 
Attila Csordas
PROVIDER: PRD000465 | Pride | 2012-07-26
REPOSITORIES: Pride
Publications
Improving reproducibility and sensitivity in identifying human proteins by shotgun proteomics.
Analytical chemistry 20040701 13
Identifying proteins in cell extracts by shotgun proteomics involves digesting the proteins, sequencing the resulting peptides by data-dependent mass spectrometry (MS/MS), and searching protein databases to identify the proteins from which the peptides are derived. Manual analysis and direct spectral comparison reveal that scores from two commonly used search programs (Sequest and Mascot) validate less than half of potentially identifiable MS/MS spectra (class positive) from shotgun analyses of ...[more]
