Dataset Information
Characterizing complex peptide mixtures using a multi-dimensional LC-MS system : Saccharomyces cerevisiae
Ontology highlight
ABSTRACT:
INSTRUMENT(S): instrument model
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)
SUBMITTER: 
Attila Csordas
PROVIDER: PRD000469 | Pride | 2012-07-26
REPOSITORIES: Pride
Publications
Characterizing complex peptide mixtures using a multi-dimensional liquid chromatography-mass spectrometry system: Saccharomyces cerevisiae as a model system.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 20041001 1
A rugged, reproducible, multi-dimensional LC-MS system was developed to identify and characterize proteins involved in protein-protein interactions and/or protein complexes. Our objective was to optimize chromatographic parameters for complex protein mixture analyses using automated peptide sequence recognition as an analytical end-point. The chromatographic system uses orthogonal separation mechanisms by employing strong cation exchange (SCX) in the first dimension and reversed phase (RP) in th ...[more]
