Dataset Information
Analysis of the periplasmic proteome of Yersinia pestis KIM6+
Ontology highlight
ABSTRACT:
INSTRUMENT(S):
ORGANISM(S): Yersinia Pestis
SUBMITTER: 
Attila Csordas
LAB HEAD: Attila Csordas
PROVIDER: PRD000494 | Pride | 2012-07-26
REPOSITORIES: Pride
Dataset's files
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Publications
Integral and peripheral association of proteins and protein complexes with Yersinia pestis inner and outer membranes.
Proteome science 20090219
Yersinia pestis proteins were sequentially extracted from crude membranes with a high salt buffer (2.5 M NaBr), an alkaline solution (180 mM Na2CO3, pH 11.3) and membrane denaturants (8 M urea, 2 M thiourea and 1% amidosulfobetaine-14). Separation of proteins by 2D gel electrophoresis was followed by identification of more than 600 gene products by MS. Data from differential 2D gel display experiments, comparing protein abundances in cytoplasmic, periplasmic and all three membrane fractions, wer ...[more]
