Ontology highlight
ABSTRACT:
INSTRUMENT(S): instrument model
ORGANISM(S): Yersinia Pestis
SUBMITTER: Attila Csordas
PROVIDER: PRD000499 | Pride | 2012-07-26
REPOSITORIES: Pride
Proteome science 20090219
Yersinia pestis proteins were sequentially extracted from crude membranes with a high salt buffer (2.5 M NaBr), an alkaline solution (180 mM Na2CO3, pH 11.3) and membrane denaturants (8 M urea, 2 M thiourea and 1% amidosulfobetaine-14). Separation of proteins by 2D gel electrophoresis was followed by identification of more than 600 gene products by MS. Data from differential 2D gel display experiments, comparing protein abundances in cytoplasmic, periplasmic and all three membrane fractions, wer ...[more]