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SILACE - Enhanced energy metabolism contributes to the extended life span of calorie-restricted Caenorhabditis elegans

ABSTRACT: WT worm is unlabeled and eat-2 knock out worm is labeled with 13C6-Lysine. Mixed 1 to 1 by wet weight. Fractionated to 20 fractions by off line 2D-LC.

INSTRUMENT(S): LTQ-Orbitrap

ORGANISM(S): Caenorhabditis Elegans

SUBMITTER: Masaru Miyagi

LAB HEAD: Masaru Miyagi

PROVIDER: PRD000517 | Pride | 2012-12-06

REPOSITORIES: Pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_18340.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_18340.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_18340.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_18341.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_18341.pride.mztab.gz	Mztab

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Publications

Enhanced energy metabolism contributes to the extended life span of calorie-restricted Caenorhabditis elegans.

Yuan Yiyuan Y Kadiyala Chandra S CS Ching Tsui-Ting TT Hakimi Parvin P Saha Sudipto S Xu Hua H Yuan Chao C Mullangi Vennela V Wang Liwen L Fivenson Elayne E Hanson Richard W RW Ewing Rob R Ewing Rob R Hsu Ao-Lin AL Miyagi Masaru M Feng Zhaoyang Z

The Journal of biological chemistry 20120718 37

Caloric restriction (CR) markedly extends life span and improves the health of a broad number of species. Energy metabolism fundamentally contributes to the beneficial effects of CR, but the underlying mechanisms that are responsible for this effect remain enigmatic. A multidisciplinary approach that involves quantitative proteomics, immunochemistry, metabolic quantification, and life span analysis was used to determine how CR, which occurs in the Caenorhabditis elegans eat-2 mutants, modifies e ...[more]

PMID: 22810224

Similar Datasets

Enhanced energy metabolism contributes to the extended life span of calorie-restricted Caenorhabditis elegans.

Project description:Caloric restriction (CR) markedly extends life span and improves the health of a broad number of species. Energy metabolism fundamentally contributes to the beneficial effects of CR, but the underlying mechanisms that are responsible for this effect remain enigmatic. A multidisciplinary approach that involves quantitative proteomics, immunochemistry, metabolic quantification, and life span analysis was used to determine how CR, which occurs in the Caenorhabditis elegans eat-2 mutants, modifies energy metabolism of the worm, and whether the observed modifications contribute to the CR-mediated physiological responses. A switch to fatty acid metabolism as an energy source and an enhanced rate of energy metabolism by eat-2 mutant nematodes were detected. Life span analyses validated the important role of these previously unknown alterations of energy metabolism in the CR-mediated longevity of nematodes. As observed in mice, the overexpression of the gene for the nematode analog of the cytosolic form of phosphoenolpyruvate carboxykinase caused a marked extension of the life span in C. elegans, presumably by enhancing energy metabolism via an altered rate of cataplerosis of tricarboxylic acid cycle anions. We conclude that an increase, not a decrease in fuel consumption, via an accelerated oxidation of fuels in the TCA cycle is involved in life span regulation; this mechanism may be conserved across phylogeny.

| S-EPMC3438970 | biostudies-literature

Skeletal muscle RBM3 expression is associated with extended lifespan in Ames Dwarf and calorie restricted mice.

Project description:RNA binding protein motif 3 (RBM3) is an RNA-binding and cold shock protein that protects myoblasts and promotes skeletal muscle hypertrophy by enhancing mRNA stability and translation. Muscle size is decreased during aging; however, it is typically delayed in models of extended lifespan such as the long-lived Ames Dwarf (df/df) mice and calorie restricted (CR) animals compared to age-matched controls. In light of the protective and anabolic effects of RBM3 in muscle, we hypothesized that RBM3 expression is higher in long-lived animal models. Young and old df/df mice, and adult and old UM-HET3 CR mice were used to test this hypothesis. Gastrocnemius muscles were harvested and protein was isolated for RBM3 protein measurements. CR induced a 1.7 and 1.3-fold elevation in RBM3 protein abundance compared to adult and old male mice fed ad libitum (AL) diets, respectively; this effect was shared between males and females. Ames dwarfism induced a 4.6 and 2.7-fold elevation in RBM3 protein abundance in young and old df/df mice compared to normal control littermates, respectively. In contrast, there was an age-associated decrease in cold-inducible RNA-binding protein (CIRP), suggesting these effects are specific for RBM3. Lastly, there was an age-associated increase in RNA degradation marker decapping enzyme 2 (DCP2) in UM-HET3 mice that was mitigated by CR. These results show that muscle RBM3 expression is correlated with extended lifespan in both df/df and CR animals. Identifying how RBM3 exerts protective effects in muscle may yield new insights into healthy aging of skeletal muscle.

| S-EPMC7902444 | biostudies-literature

Comparison between calorie-restricted and time-restricted without calorie restriction

Project description:Recently, it has been shown that fasting for a portion of each day has metabolic benefits, and promotes lifespan. These findings complicate the interpretation of rodent CR studies, which typically feed the animals only once per day; as they rapidly consume their food, such a regimen collaterally imposes fasting.

2021-08-16 | GSE168262 | GEO

Normal Calorie Restricted

Project description:Normal female Ames mouse (+/+ or +/df) with ad libitum access to water and fed 30% less of the standard pelleted diet with respect to the amount consumed by normal female ad libitum fed +/+ and +/df mice (LabDiet, PMI Feeds, Inc., St Louis, MO) caged with microisolator filter tops. Mice were killed between 24 to 26 months of age, tissues removed, rapidly frozen on dry ice, and stored in liquid nitrogen. Total liver RNA was isolated from frozen tissue as described (T. Tsuchiya, J.M. Dhahbi, X. Cui, P.L. Mote, A. Bartke, S.R. Spindler, Physiological Genomics, Submitted). mRNA levels were measured using the Affymetrix mouse U74Av2 array according to standard protocols. After hybridization, arrays were scanned using a Hewlett-Packard GeneArray Scanner. Image analysis was performed as described (Cao SX, Dhahbi JM, Mote PL, and Spindler SR. Genomic profiling of short- and long-term caloric restriction effects in the liver of aging mice. Proc Natl Acad Sci U S A 98: 10630-10635, 2001). A more detailed description of the methods can be found in (T. Tsuchiya, J.M. Dhahbi, X. Cui, P.L. Mote, A. Bartke, S.R. Spindler, Physiological Genomics, Submitted). Keywords = Ames dwarf Keywords = mouse Keywords = Affymetrix Keywords = caloric restriction Keywords: parallel sample

2008-03-19 | GSE1089 | GEO

Dwarf Calorie Restricted

Project description:Female Ames dwarf mouse (df/df) with ad libitum access to water and fed 30% less of the standard pelleted diet with respect to the amount consumed by female ad libitum fed df/df mice (LabDiet, PMI Feeds, Inc., St Louis, MO) caged with microisolator filter tops. Mice were killed between 24 to 26 months of age, tissues removed, rapidly frozen on dry ice, and stored in liquid nitrogen. Total liver RNA was isolated from frozen tissue as described (T. Tsuchiya, J.M. Dhahbi, X. Cui, P.L. Mote, A. Bartke, S.R. Spindler, Physiological Genomics, Submitted). mRNA levels were measured using the Affymetrix mouse U74Av2 array according to standard protocols. After hybridization, arrays were scanned using a Hewlett-Packard GeneArray Scanner. Image analysis was performed as described (Cao SX, Dhahbi JM, Mote PL, and Spindler SR. Genomic profiling of short- and long-term caloric restriction effects in the liver of aging mice. Proc Natl Acad Sci U S A 98: 10630-10635, 2001). A more detailed description of the methods can be found in (T. Tsuchiya, J.M. Dhahbi, X. Cui, P.L. Mote, A. Bartke, S.R. Spindler, Physiological Genomics, Submitted). Keywords = Ames dwarf Keywords = mouse Keywords = Affymetrix Keywords = caloric restriction Keywords: parallel sample

2005-01-10 | GSE1087 | GEO

Calorie Restricted and Non-Restricted Yeast

Project description:This SuperSeries is composed of the SubSeries listed below.

2014-09-15 | GSE53721 | GEO

Calorie Restricted and Non-Restricted Yeast

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-09-15 | E-GEOD-53721 | biostudies-arrayexpress

Comparison between calorie-restricted and time-restricted without calorie restriction

Project description:Comparison between calorie-restricted and time-restricted without calorie restriction

| PRJNA706697 | ENA

786-O ubiquitiome - Loss of BAP1 Results in Growth Inhibition and Enhances Mesenchymal-Epithelial Transition in Kidney Tumor Cells

Project description:786-O WT and BAP1-KO cells were cultured in heavy (13C6-L-arginine, 13C6-L-lysine, Cambridge Isotope Lab) and light (unlabeled L-arginine and L-lysine) SILAC RPMI-1640 medium (Thermo Fisher Scienific), respectively. After more than 10 passages, cells were lysed with 8 M urea and 5 mg protein from each group were mix. The ubiquitinated peptide enrichment was performed using PTMScan® (Cell Signaling Technology, #5562) following the manufacturer’s instructions.

2019-04-18 | PXD013127 | Pride

Structural modulation of gut microbiota in life-long calorie-restricted mice.

Project description:Calorie restriction has been regarded as the only experimental regimen that can effectively lengthen lifespan in various animal models, but the actual mechanism remains controversial. The gut microbiota has been shown to have a pivotal role in host health, and its structure is mostly shaped by diet. Here we show that life-long calorie restriction on both high-fat or low-fat diet, but not voluntary exercise, significantly changes the overall structure of the gut microbiota of C57BL/6 J mice. Calorie restriction enriches phylotypes positively correlated with lifespan, for example, the genus Lactobacillus on low-fat diet, and reduces phylotypes negatively correlated with lifespan. These calorie restriction-induced changes in the gut microbiota are concomitant with significantly reduced serum levels of lipopolysaccharide-binding protein, suggesting that animals under calorie restriction can establish a structurally balanced architecture of gut microbiota that may exert a health benefit to the host via reduction of antigen load from the gut.

| S-EPMC3717500 | biostudies-literature

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