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Identification of C6-ceramide-interacting proteins in D6P2T Schwannoma cells.

ABSTRACT: In this study, we have utilized affinity purification with a synthetic biotin-ceramide and LC-MS/MS to identify potential ceramide-interacting proteins in D6P2T Schwannoma cells.

INSTRUMENT(S): instrument model, LTQ

ORGANISM(S): Rattus Rattus (black Rat)

TISSUE(S): Rt4-d6p2t Cell

SUBMITTER: Venkatesh Kota

PROVIDER: PRD000566 | Pride | 2015-09-07

REPOSITORIES: Pride

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Identification of C(6) -ceramide-interacting proteins in D6P2T Schwannoma cells.

Kota Venkatesh V Szulc Zdzislaw M ZM Hama Hiroko H

Proteomics 20120701 13

Ceramide is a bioactive molecule involved in numerous cell signaling pathways that are associated with cell cycle control, differentiation, senescence, and apoptosis. Although substantial knowledge about ceramide-regulated pathways has accumulated in the past decade, molecular mechanisms of ceramide action remain poorly understood, primarily due to limited information about ceramide-binding proteins. In the present study, we used affinity purification with a synthetic biotin-conjugated C(6) -cer ...[more]

PMID: 22623228

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