Dataset Information

Tyrosine phosphorylation in Arabidopsis

ABSTRACT: Quantification of pY abundance changes in Arabidopsis upon Flg22 stimulation

INSTRUMENT(S): LTQ-Orbitrap

ORGANISM(S): Arabidopsis

SUBMITTER: Paul Boersema

LAB HEAD: Paul Boersema

PROVIDER: PRD000577 | Pride | 2015-06-02

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_19496.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_19496.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_19496.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_19497.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_19497.pride.mztab.gz	Mztab

Items per page:

1 - 5 of 9

Publications

Targeted quantitative phosphoproteomics approach for the detection of phospho-tyrosine signaling in plants.

Mithoe Sharon C SC Boersema Paul J PJ Berke Lidija L Snel Berend B Heck Albert J R AJ Menke Frank L H FL

Journal of proteome research 20111201 1

Tyrosine (Tyr) phosphorylation plays an essential role in signaling in animal systems. However, a few studies have also reported Tyr phosphorylation in plants, but the relative contribution of tyrosine phosphorylation to plant signal transduction has remained an open question. We present an approach to selectively measure and quantify Tyr phosphorylation in plant cells, which can also be applied to whole plants. We combined a (15)N stable isotope metabolic labeling strategy with an immuno-affini ...[more]

PMID: 22074104

Similar Datasets

Project description:In this research a high-throughput RNA sequencing based transcriptome analysis technique (RNA-Seq) was used to evaluate differentially expressed genes (DEGs) in the wild type Arabidopsis seedling in response to flg22, a well-known microbe-associated molecular patterns (MAMP), and AtPep1, a well-known peptide representing an endogenous damage-associated molecular patterns (DAMP). The results of our study revealed that 1895 (1634 up-regulated and 261 down-regulated) and 2271 (1706 up-regulated and 565 down-regulated) significant differentially expressed genes in response to flg22 and AtPep1 treatment, respectively. Among significant DEGs, we observed that a number of hitherto overlooked genes have been found to be induced upon treatment with either flg22 or with AtPep1, indicating their possible involvement in innate immunity. Here, we characterized two of them, namely PP2-B13 and ACLP1. PP2-B13 contains an F-box domain and shows similarity to carbohydrate binding proteins. ACLP1 is a protein of unknown function with highest similarity to actin cross linking proteins and includes a fascin domain. Using qPCR, we verified that the genes encoding PP2-B13, and ACLP1 were highly induced upon treatment of leaf disks with flg22. We obtained T-DNA insertion mutants and generated homozygous mutant lines. None of the mutants showed a phenotype in the absence of infection. pp2-b13 and aclp1 mutants showed an increased susceptibility to infection by the virulent pathogen Pseudomomas syringae pv tomato mutant hrcC-, as evidenced by an increased growth of the pathogen in planta. Further we present evidence that aclp1 was deficient in ethylene production upon flg22 treatment, while pp2-b13, was deficient in ROS production. In conclusion, the products of these genes contribute to plant immunity against bacterial pathogens, although there is currently no clue for their mechanism of action. The results from this research provide new information to a better understanding of the immune system in Arabidopsis.