Proteomics

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Various Fractionation Methods - Expanding the zebrafish embryo proteome using multiple fractionation approaches and tandem mass spectrometry

ABSTRACT: Zebrafish Embryo Proteome

INSTRUMENT(S): instrument model, LTQ Velos

ORGANISM(S): Danio Rerio (zebrafish) (brachydanio Rerio)

SUBMITTER: Rachel Li  

PROVIDER: PRD000595 | Pride | 2014-05-01

REPOSITORIES: Pride

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Publications

Expanding the zebrafish embryo proteome using multiple fractionation approaches and tandem mass spectrometry.

Lößner Christopher C   Wee Sheena S   Ler Siok Ghee SG   Li Rachel H X RH   Carney Tom T   Blackstock Walter W   Gunaratne Jayantha J  

Proteomics 20120601 11

The proteome of zebrafish, Danio rerio, embryos has not been studied in great detail mainly due to the presence of high abundance yolk proteins in embryos. Here we report the highest number of the zebrafish embryo proteins identified so far to our knowledge, through a combination of a protein-level fractionation approach (1D SDS-PAGE) and two different peptide-level fractionation approaches (IEF and strong anion exchange (SAX)) of deyolked zebrafish embryos followed by LC-MS/MS. We detected 5267  ...[more]