Name: Axon degeneration study - Soluble axoplasm enriched from injured CNS axons reveals the early modulation of the actin cytoskeleton
Keywords: Not available

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Axon degeneration study - Soluble axoplasm enriched from injured CNS axons reveals the early modulation of the actin cytoskeleton

ABSTRACT: 4-plex iTRAQ analysis of crushed vs naive optic nerve

INSTRUMENT(S): instrument model, Q-Tof ultima

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Optic Nerve

SUBMITTER: Patrick Garland

PROVIDER: PRD000606 | Pride | 2013-06-11

REPOSITORIES: Pride

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Soluble axoplasm enriched from injured CNS axons reveals the early modulation of the actin cytoskeleton.

Garland Patrick P Broom Lucy J LJ Quraishe Shmma S Dalton Paul D PD Skipp Paul P Newman Tracey A TA Perry V Hugh VH

PloS one 20121024 10

Axon injury and degeneration is a common consequence of diverse neurological conditions including multiple sclerosis, traumatic brain injury and spinal cord injury. The molecular events underlying axon degeneration are poorly understood. We have developed a novel method to enrich for axoplasm from rodent optic nerve and characterised the early events in Wallerian degeneration using an unbiased proteomics screen. Our detergent-free method draws axoplasm into a dehydrated hydrogel of the polymer p ...[more]

PMID: 23115653

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