Dataset Information

Surfome of different form cells of C. albicans. LJ Chaffin and C. Gil

ABSTRACT: Identification of exposed surface proteins of biofilms, hyphae and yeast form cells of Candida albicans. In vivo trypsin digestion and LC-MS protein identification.

INSTRUMENT(S): instrument model, 4800 Proteomics Analyzer

ORGANISM(S): Candida Albicans Sc5314

TISSUE(S): Hyphal Cell, Yeast, Biofilm

SUBMITTER: Vital Vialas

PROVIDER: PRD000640 | Pride | 2012-10-09

REPOSITORIES: Pride

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Publications

Cell surface shaving of Candida albicans biofilms, hyphae, and yeast form cells.

Vialás Vital V Perumal Palani P Gutierrez Dolores D Ximénez-Embún Pilar P Nombela César C Gil Concha C Chaffin W LaJean WL

Proteomics 20120801 14

We used a brief trypsin treatment followed by peptide separation and identification using nano-LC followed by off-line MS/MS to identify the surface proteins on live Candida albicans organisms growing in biofilms and planktonic yeast cells and hyphae. One hundred thirty-one proteins were present in at least two of the three replicates of one condition and distributed in various combinations of the three growth conditions. Both previously reported and new surface proteins were identified and thes ...[more]

PMID: 22685022

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Project description:Candida spp. are commensal opportunistic fungal pathogens that often colonize and infect mucosal surfaces of the human body. Candida, along with other microbes in the microbiota, generally grow as biofilms in a polymicrobial environment. Due to the nature of cellular growth in a biofilm (such as production of a protective extracellular matrix) and the recalcitrance of biofilms, infections involving biofilms are very difficult to treat with antibiotics and perpetuate the cycle of infection. The two most commonly isolated Candida spp. from Candida infections are Candida albicans and Candida glabrata, and the presence of both of these species results in increased patient inflammation and overall biofilm formation. This work aims to investigate the interspecies interactions between C. albicans (Ca) and C. glabrata (Cg) in co-culture through transcriptome analysis over the course of biofilm growth. We report that during co-culture, lipid biosynthesis and transporter genes were significantly modulated in both Ca and Cg. Differentially expressed genes in Ca during co-culture growth included putative transporter genes (C2_02180W_A and C1_09210C_B; up-regulated), amino acid biosynthesis (ARO7; up-regulated most in Ca:Cg 1:3), and lipid-related genes (LIP3 and IPT1; down-regulated). Differentially expressed genes in Cg in co-culture included putative transmembrane transporters (CAGL0H03399g and CAGL0K04609g; up-regulated), an oxidative stress response gene (CAGL0E04114g; down-regulated most in Ca:Cg 1:3), genes involved in the TCA cycle (LYS12 and CAGL0J06402g; down-regulated), and several genes involved in cell wall/membrane biosynthesis (SEC53, GAS2, VIG9; down-regulated). Additionally, confocal microscopy was utilized for membrane lipid analysis between monoculture and co-culture biofilms. Through filipin-stained lipid analysis, we found that there was a significant increase in cell membrane lipid content in Ca:Cg 1:3 biofilms compared to Ca and Ca:Cg 3:1 biofilms. These results suggest substantial modifications of both cell wall, cell membrane, and transporters in both Ca and Cg during the time course of co-culture growth, which allows for increased biofilm formation and virulence in Candida co-culture biofilms.

Dataset Information

Surfome of different form cells of C. albicans. LJ Chaffin and C. Gil

Publications

Cell surface shaving of Candida albicans biofilms, hyphae, and yeast form cells.

Similar Datasets

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