Proteomics

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Label free nano-LC MALDI-TOF-MS/MS analysis of Triton X-100 solubilized proteins from bovine brain capillary endothelial cells (BCECs).

ABSTRACT: Triton X-100 soluble crude extract from BCECs displaying limited blood-brain barrier functions was fractionated into 5 fractions (F0, F25, F50, F75) with increasing concentrations of acetonitrile (0%, 25%, 50%, 75%). This is the merging of triplicate dataset of subfraction # F0.

INSTRUMENT(S): Bruker Daltonics flex series, instrument model

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Brain, Capillary Endothelial Cell

SUBMITTER: Christophe FLAHAUT  

PROVIDER: PRD000681 | Pride | 2012-11-27

REPOSITORIES: Pride

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Publications

An easier, reproducible, and mass-production method to study the blood-brain barrier in vitro.

Dehouck M P MP   Méresse S S   Delorme P P   Fruchart J C JC   Cecchelli R R  

Journal of neurochemistry 19900501 5

To provide an "in vitro" system for studying brain capillary function, we have developed a process of coculture that closely mimics the "in vivo" situation by culturing brain capillary endothelial cells on one side of a filter and astrocytes on the other. Under these conditions, endothelial cells retain all the endothelial cell markers and the characteristics of the blood-brain barrier, including tight junctions and gamma-glutamyl transpeptidase activity. The average electric resistance for the  ...[more]

PMID: 2182777

Publication: 1/4

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