Browse
Submit Data
Databases
API
Help

Dataset Information

406 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human MNC proteome - Proteome profiling of peripheral mononuclear cells from human blood

ABSTRACT: Proteome characterization of mononuclear cells extracted from human blood - Dataset 3

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Mononuclear Cell, Blood

SUBMITTER: Daniel Martins-de-Souza

PROVIDER: PRD000732 | Pride | 2013-01-03

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Publications

Proteome profiling of peripheral mononuclear cells from human blood.

Maccarrone Giuseppina G Rewerts Christiane C Lebar Maria M Turck Christoph W CW Martins-de-Souza Daniel D

Proteomics 20130124 5

Peripheral blood mononuclear cells (MNCs) are accessible through blood collection and represent a useful source for investigations on disease mechanisms and treatment response. Aiming to build a reference proteome database, we generated three proteome data sets from MNCs using a combination of SDS-PAGE and nanoflow LC-MS. Experiments were performed in triplicates and 514 unique proteins were identified by at least two non-redundant peptides with 95% confidence for all replicates. Identified prot ...[more]

PMID: 23281267

Similar Datasets

Proteome signatures of inflammatory activated primary human peripheral blood mononuclear cells

Project description:Primary human peripheral blood mononuclear cells were isolated from full blood by standard ficoll centrifugation. Cells were washed and processed immediately.

2012-07-16 | PRD000731 | Pride

Microarray profiling peripheral blood mononuclear cells

Project description:Microarray profiling peripheral blood mononuclear cells

| PRJNA418401 | ENA

The Impact of Blood-Processing Time on the Proteome of Human Peripheral Blood Mononuclear Cells

Project description:Human peripheral blood mononuclear cells (PBMC) are key to several diagnostics assays and basic science research. Blood pre-analytical variations that occur before obtaining the PBMC fraction can have a significant impact on the results of the assays, including viability, composition, integrity, and gene expression changes of immune cells. With this as motivation, we performed a quantitative shotgun proteomics analysis using Isobaric Tag for Relative and Absolute Quantitation (iTRAQ 8plex) labeling to compare PBMC obtained from fresh versus 24h-stored blood at room temperature. We identified 3,195 proteins, of which 245 were differentially abundant. Our results revealed enriched pathways in the fresh-PBMC samples related to exocytosis, localization, vesicle-mediated transport, cell activation, and secretion. In contrast, pathways related to exocytosis, neutrophil degranulation and activation, granulocyte activation, leukocyte degranulation, and myeloid leukocyte activation involved in immune response were enriched in the 24h-PBMC samples, which may indicate probable granulocyte contamination and activation due to blood storage time and temperature. Examples of upregulated proteins in the 24h-PBMC sample are: CAMP, S100A8, LTA4H, RASAL3, and S100A6, which are involved in an adaptive immune system and antimicrobial activity, proinflammatory mediation, aminopeptidase activities, and naïve T cells survival. Moreover, examples of downregulated proteins are: NDUFA5, TAGLN2, H3C1, TUBA8, and CCT2 that are related to the cytoskeleton, cell junction, mitochondrial respiratory chain. In conclusion, blood-processing time directly impacts the proteomic profile of human PBMC.

2020-12-14 | PXD021367 | Pride

Real-time quantitative PCR analysis of human peripheral mononuclear cells (PBMC) [mifinder Phenotypes]

Project description:Human peripheral mononuclear cells were purified from control and diseased patient volunteers blood using ficol and miRNA was extracted using QIAGEN miRNeasy kit

2020-10-27 | GSE135659 | GEO

The effects of Zoledronate on human peripheral blood mononuclear cells

Project description:Zoledronate is well known to act on osteoclasts to inhibit bone resorption.Zoledronate is administrated intravenously in clinical use, however, actions of zoledronate on cells in blood circulation are not fully understood. To understand its actions of human peripheral blood mononuclear cells (h-PBMC), we performed gene expression profiling in zoledronate-treated h-PBMC.

2019-10-10 | GSE121063 | GEO

Single cell profiling of peripheral blood mononuclear cells from healthy human donor

Project description:Healthy human peripheral blood mononuclear cells (PBMCs) investigated with 10x Genomics single cell RNA-sequencing.

2018-07-28 | GSE115189 | GEO

Real-time quantitative PCR analysis of human peripheral mononuclear cells (PBMC) [mifinder Stages]

Project description:Human peripheral mononuclear cells were purified from control and diseased patient (Psedoexfoliation (PXF), Ocular Hypertension (OHT), Psedoexfoliation Glaucoma (PXG)) volunteers blood using ficol and miRNA was extracted using QIAGEN miRNeasy kit

2020-10-27 | GSE135660 | GEO

Transcriptome profiling of peripheral blood mononuclear cell subsets

Project description:Transcriptome profiling was conducted on 2 replicate samples of total peripheral blood mononuclear cells (PBMC) and isolated PBMC subsets.

2020-04-14 | GSE101489 | GEO

Real-time quantitative PCR analysis of human peripheral mononuclear cells (PBMC) [miRNA Fibrosis Phenotypes]

Project description:Human peripheral mononuclear cells were purified from control and diseased (Cataract, Classical PXF, Pigmentary PXF) patient volunteers blood using ficol and miRNA was extracted using QIAGEN miRNeasy kit

2020-10-27 | GSE135657 | GEO

Human healthy mononuclear blood cells

Project description:Healthy mononuclear cells from periferal blood (PB), mobilized peripheral blood (MPB) or bone marrow (BM) of healthy adult donors.

2023-07-31 | GSE132720 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data