Proteomics

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Proteomic Analysis of Post-hatch Bursa of Fabricius

ABSTRACT: Duplicate samples of bursal B- and stroma cells were isolated from four 21-day-old Ross 508 chickens as described (McCarthy, Burgess et al, 2005) except that we kept the stromal cells that do not pass through sterile gauze. Samples were analysed by DDF-MudPIT and the resulting spectra searched against an avian subset (AvDb; search term: gallus; 01/15/05) of the NRPD (NCBI) using Bioworks Browser 3.2 (ThermoElectron) as described (McCarthy, Burgess et al, 2005). The peptide (MS precursor ion) mass tolerance was set to 1.5 Da and the fragment ion (MS2) mass tolerance was set to 1.0 Da. Peptide matches were considered genuine if they had â??Cn â?¥ 0.1 and X correlation â?¥ 1.9 (+1), 2.2 (+2), 3.75 (+3).

INSTRUMENT(S): instrument model, LCQ Deca XP Plus

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Bursa Of Fabricius

SUBMITTER: DAVE THORNEYCROFT  

PROVIDER: PRD000755 | Pride | 2005-08-21

REPOSITORIES: Pride

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Publications

Differential detergent fractionation for non-electrophoretic eukaryote cell proteomics.

McCarthy Fiona M FM   Burgess Shane C SC   van den Berg Bart H J BH   Koter Marek D MD   Pharr G Todd GT  

Journal of proteome research 20050301 2

Differential detergent fractionation (DDF), which relies on detergents to sequentially extract proteins from eukaryotic cells, has been used to increase proteome coverage of 2D-PAGE. Here, we used DDF extraction in conjunction with the nonelectrophoretic proteomics method of liquid chromatography and electrospray ionization tandem mass spectrometry. We demonstrate that DDF can be used with 2D-LC ESI MS2 for comprehensive cellular proteomics, including a large proportion of membrane proteins. Com  ...[more]

PMID: 15822906

Publication: 1/2

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