Proteomic Analysis of Post-hatch Bursa of Fabricius

ABSTRACT: Duplicate samples of bursal B- and stroma cells were isolated from four 21-day-old Ross 508 chickens as described (McCarthy, Burgess et al, 2005) except that we kept the stromal cells that do not pass through sterile gauze. Samples were analysed by DDF-MudPIT and the resulting spectra searched against an avian subset (AvDb; search term: gallus; 01/15/05) of the NRPD (NCBI) using Bioworks Browser 3.2 (ThermoElectron) as described (McCarthy, Burgess et al, 2005). The peptide (MS precursor ion) mass tolerance was set to 1.5 Da and the fragment ion (MS2) mass tolerance was set to 1.0 Da. Peptide matches were considered genuine if they had â??Cn â?¥ 0.1 and X correlation â?¥ 1.9 (+1), 2.2 (+2), 3.75 (+3).

INSTRUMENT(S):

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Bursa Of Fabricius

SUBMITTER: DAVE THORNEYCROFT  

LAB HEAD: DAVE THORNEYCROFT

PROVIDER: PRD000755 | Pride | 2005-08-21

REPOSITORIES: Pride