Global liver proteomics of rats exposed for five days to phenobarbital identifies changes associated with cancer and with cyp metabolism

ABSTRACT: Adult male Sprague Dawley-derived rats were housed and fed as described previously (Dail et al., 2007). Three rats were treated i.p. with PB in saline at 80 mg/kg body weight/day (treated) for 5 days as described previously (Ma and Chambers; 1995). Following sacrifice on day 5, the livers were removed and stored at -80 C as previously described (Dail et al., 2007). Five 8 µm sections were taken from each of the three control and three experimental livers resulting in fifteen sections from each group using a Triangle Biomedical Supply Co Minotome Plus (Durham, NC) microtome. Differential detergent fractionation two dimensional liquid chromatography electrospray ionization tandem mass spectrometry (DDF 2D LC ESI MS2) and systems biology modeling were used to identify alterations in toxicologically relevant hepatic molecular functions and biological processes in the livers of rats

INSTRUMENT(S): LCQ Deca XP Plus

ORGANISM(S): Rattus Norvegicus (rat)

SUBMITTER: Shane Burgess  

LAB HEAD: Shane Burgess

PROVIDER: PRD000797 | Pride | 2008-12-09

REPOSITORIES: Pride