Dataset Information

Proteomics analysis of Thermoplasma acidophilum with molecular sieve chromatography in combination with LC-MS/MS

ABSTRACT: Not available

INSTRUMENT(S): LCQ-DECA XP plus ion trap mass spectrometer

ORGANISM(S): Thermoplasma Acidophilum Dsm 1728

SUBMITTER: Istvan Nagy

LAB HEAD: Istvan Nagy

PROVIDER: PRD000803 | Pride | 2009-07-28

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_9163.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_9163.xml.gz	Xml

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Publications

Proteomics analysis of Thermoplasma acidophilum with a focus on protein complexes.

Sun Na N Beck Florian F Knispel Roland Wilhelm RW Siedler Frank F Scheffer Beatrix B Nickell Stephan S Baumeister Wolfgang W Nagy István I

Molecular & cellular proteomics : MCP 20061206 3

Two-dimensional gel electrophoresis (2DE) and MALDI-TOF MS were used to obtain a global view of the cytoplasmic proteins expressed by Thermoplasma acidophilum. In addition, glycerol gradient ultracentrifugation coupled to 2DE-MALDI-TOF MS analysis was used to identify subunits of macromolecular complexes. With the 2DE proteomics approach, over 900 spots were resolved of which 271 proteins were identified. A significant number of these form macromolecular complexes, among them the ribosome, prote ...[more]

PMID: 17151018

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