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Proteomic analysis of the fish pathogen Flavobacterium columnare

ABSTRACT: Clinical Flavobacterium columnare ATCC 49512 was grown on Flavobacterium columnare growth medium (FCGM). Bacteria from four colonies at mid-exponential phase were harvested, total proteins were isolated, and identified using 2-DE MALDI TOF/TOF MS and 2-D LC ESI MS/MS analyses. The MS/MS spectra for all peptides were analyzed using sequest algorithm

INSTRUMENT(S): instrument model, LCQ Deca XP Plus

ORGANISM(S): Flavobacterium Columnare

SUBMITTER: PRADEEP KUMAR DUMPALA

PROVIDER: PRD000810 | Pride | 2016-03-11

REPOSITORIES: Pride

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Proteomic analysis of the fish pathogen Flavobacterium columnare.

Dumpala Pradeep R PR Gülsoy Nagihan N Lawrence Mark L ML Karsi Attila A

Proteome science 20100604

<h4>Background</h4>Flavobacterium columnare causes columnaris disease in cultured and wild fish populations worldwide. Columnaris is the second most prevalent bacterial disease of commercial channel catfish industry in the United States. Despite its economic importance, little is known about the expressed proteins and virulence mechanisms of F. columnare. Here, we report the first high throughput proteomic analysis of F. columnare using 2-D LC ESI MS/MS and 2-DE MALDI TOF/TOF MS.<h4>Results</h4> ...[more]

PMID: 20525376

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