Proteomic screen reveals Fbw7 as a modulator of the NF-kB pathway

ABSTRACT: Proteomics on HCT116 cells. 3 samples wild-type, 3 samples Fbw7 KO. Subcellular fractionation to cytoplasmic and nuclear fractions. Proteins were reduced with DTT, alkylated with iodoacetamide, digested with trypsin, and labelled with TMT6-plex: 126,127,128 - wildtype; 129,130,131 - Fbw7 KO. Sample separation by high resolution isoelectric focusing at peptide level, whereafter 72 fractions were loaded onto reversed phase LC-MSMS

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Rui Branca  

LAB HEAD: Rui Branca

PROVIDER: PXD000005 | Pride | 2012-06-29

REPOSITORIES: Pride