Comprehensive mapping of the bull sperm surface proteome

ABSTRACT: Nitrogen cavitation of bovine sperm to isolate plasma membrane. FASP digestion using trypsin. Data acquired using 5 workflows: (1) Unfractionated on a 5600 TripleTOF; (2) SCX-ESI-LC-MS/MS on a QSTAR Elite; (3) 1D Gel-LC-ESI-LC-MS/MS on a QSTAR Elite; (4) SCX-LC-MALDI-MS/MS on a UltrafleXtreme; (5) 1D Gel-LC-MALDI-MS/MS on a UltrafleXtreme

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Spermatozoon

SUBMITTER: Michelle Colgrave  

LAB HEAD: Michael K. Holland

PROVIDER: PXD000007 | Pride | 2020-01-27

REPOSITORIES: Pride