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Omics score: 123

IQUARI - Integral quantification accuracy estimation for reporter ion-based quantitative proteomics (iQuARI)

ABSTRACT: Study on the MS1 interference problem for reporter ion quantification using orthogonal proteomes: human and pyrococcus furiosus.

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Pyrococcus Furiosus Homo Sapiens (human)

TISSUE(S): Blood Platelet, Platelet, Permanent Cell Line Cell, Whole Body, Hela Cell

SUBMITTER: Marc Vaudel

PROVIDER: PXD000013 | Pride | 2012-08-13

REPOSITORIES: Pride

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Integral quantification accuracy estimation for reporter ion-based quantitative proteomics (iQuARI).

Vaudel Marc M Burkhart Julia M JM Radau Sonja S Zahedi René P RP Martens Lennart L Sickmann Albert A

Journal of proteome research 20120830 10

With the increasing popularity of comparative studies of complex proteomes, reporter ion-based quantification methods such as iTRAQ and TMT have become commonplace in biological studies. Their appeal derives from simple multiplexing and quantification of several samples at reasonable cost. This advantage yet comes with a known shortcoming: precursors of different species can interfere, thus reducing the quantification accuracy. Recently, two methods were brought to the community alleviating the ...[more]

PMID: 22874012

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