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The metabolic regulation of sporulation and parasporal crystal formation in Bacillus thuringiensis revealed by transcriptomics and proteomics

ABSTRACT: Proteomics on B. thuringiensis CT_43 cells in GYS medium. Two biological replicate cell samples were collected at time points of 7 h, 9 h, 13 h and 22 h, respectively. The crude proteins were purified using the ReadyPrep 2-D Cleanup Kit, underwent the reductive alkylation, tryptically digested, and were labeled with 8-plex iTRAQ reagents as follows: 7 h-1, 113; 7 h-2, 114; 9 h-1, 115; 9 h-2, 116; 13 h-1, 117; 13 h-2, 118; 22 h-1, 119; and 22 h-2, 121. The labeled samples were pooled and resolved into 12 fractions, which were loaded onto LC-MSMS.

INSTRUMENT(S): instrument model, TripleTOF 5600

ORGANISM(S): Escherichia Coli O157:nm

SUBMITTER: Jieping Wang

LAB HEAD: Jin He

PROVIDER: PXD000020 | Pride | 2019-11-22

REPOSITORIES: Pride

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The metabolic regulation of sporulation and parasporal crystal formation in Bacillus thuringiensis revealed by transcriptomics and proteomics.

Wang Jieping J Mei Han H Zheng Cao C Qian Hongliang H Cui Cui C Fu Yang Y Su Jianmei J Liu Ziduo Z Yu Ziniu Z He Jin J

Molecular & cellular proteomics : MCP 20130212 5

Bacillus thuringiensis is a well-known entomopathogenic bacterium used worldwide as an environmentally compatible biopesticide. During sporulation, B. thuringiensis accumulates a large number of parasporal crystals consisting of insecticidal crystal proteins (ICPs) that can account for nearly 20-30% of the cell's dry weight. However, the metabolic regulation mechanisms of ICP synthesis remain to be elucidated. In this study, the combined efforts in transcriptomics and proteomics mainly uncovered ...[more]

PMID: 23408684

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