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Omics score: 93

Analysis of E2F2 interacting proteins

ABSTRACT: In an attempt to identify proteins interacting with transcription factor E2F2, proteins extracted from HA-E2F2 overexpressing HEK293T cells were were subjected to immunoprecipitation (IP) with an anti-HA antibody or, as a negative control (NegCt), with an anti-T antibody. Immunoprecipitated proteins were eluted, digested with trypsin, and the resulting peptides were analyzed by LC-MS/MS. The whole procedure was repeated several times in an attempt to gauge reproducibility (nine replicates of the anti-HA IP and six replicates of the control IP).

INSTRUMENT(S): instrument model, Q-Tof micro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293t Cell, Kidney Cell

SUBMITTER: Nerea Osinalde

PROVIDER: PXD000026 | Pride | 2013-01-18

REPOSITORIES: Pride

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The nuclear protein ALY binds to and modulates the activity of transcription factor E2F2.

Osinalde Nerea N Olea Miguel M Mitxelena Jone J Aloria Kerman K Rodriguez Jose Antonio JA Fullaondo Asier A Arizmendi Jesus M JM Zubiaga Ana M AM

Molecular & cellular proteomics : MCP 20130107 5

E2F transcription factors control the expression of genes involved in a variety of essential cellular processes and consequently their activity needs to be tightly regulated. Protein-protein interactions are thought to be key modulators of E2F activity. To gain insight into the mechanisms that regulate the activity of E2F2, we searched for novel proteins that associate with this transcription factor. We show that the nuclear protein ALY (THO complex 4), originally described as a transcriptional ...[more]

PMID: 23297349

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Analysis of E2F2 interacting proteins

Project description:In an attempt to identify proteins interacting with transcription factor E2F2, proteins extracted from HA-E2F2 overexpressing HEK293T cells were were subjected to immunoprecipitation (IP) with an anti-HA antibody or, as a negative control (NegCt), with an anti-T antibody. Immunoprecipitated proteins were eluted, digested with trypsin, and the resulting peptides were analyzed by LC-MS/MS. The whole procedure was repeated several times in an attempt to gauge reproducibility (nine replicates of the anti-HA IP and six replicates of the control IP).

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