Proteomics,Multiomics

Dataset Information

88

Hrp3-TAP_Purification from Schizosaccharomyces pombe


ABSTRACT: Hrp3_Purification from Schizosaccharomyces pombe 972h- Eukaryotic genome is composed of repeating units of nucleosomes to form chromatin arrays. A canonical gene is marked by nucleosome free region (NFR) at its 5’ end followed by uniformly spaced arrays of nucleosomes. In fission yeast we show both biochemically and in vivo that both Hrp1 and Hrp3 are key determinants of uniform spacing of genic arrays.

OTHER RELATED OMICS DATASETS IN: PRJNA177319

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Schizosaccharomyces Pombe 972h-

SUBMITTER: Ignasi Forne  

PROVIDER: PXD000041 | Pride | 2014-05-16

REPOSITORIES: Pride

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Publications

CHD1 remodelers regulate nucleosome spacing in vitro and align nucleosomal arrays over gene coding regions in S. pombe.

Pointner Julia J   Persson Jenna J   Prasad Punit P   Norman-Axelsson Ulrika U   Strålfors Annelie A   Khorosjutina Olga O   Krietenstein Nils N   Svensson J Peter JP   Ekwall Karl K   Korber Philipp P  

The EMBO journal 20121026 23


Nucleosome positioning governs access to eukaryotic genomes. Many genes show a stereotypic organisation at their 5'end: a nucleosome free region just upstream of the transcription start site (TSS) followed by a regular nucleosomal array over the coding region. The determinants for this pattern are unclear, but nucleosome remodelers are likely critical. Here we study the role of remodelers in global nucleosome positioning in S. pombe and the corresponding changes in expression. We find a striking  ...[more]

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