Dataset Information

Proteomic analysis of intact flagella of procyclic Trypanosoma brucei cells identifies novel flagellar proteins with unique sub-localisation and dynamics

ABSTRACT: Identification of intact flagella of procyclic Trypanosoma brucei cells by LC-MS/MS after a trypsin shaving

INSTRUMENT(S):

ORGANISM(S): Trypanosoma Brucei

SUBMITTER: Philippe Bastin

LAB HEAD: Philippe Bastin

PROVIDER: PXD000051 | Pride | 2018-12-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20100729_Bastin_Culot_FR_24h.raw	Raw
	20100729_Bastin_Culot_FR_48h.raw	Raw
	20100729_Bastin_Culot_FR_72h.raw	Raw
	20100729_Bastin_Eluat_FR_24h.raw	Raw
	20100729_Bastin_Eluat_FR_48h.raw	Raw

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Publications

Proteomic analysis of intact flagella of procyclic Trypanosoma brucei cells identifies novel flagellar proteins with unique sub-localization and dynamics.

Subota Ines I Julkowska Daria D Vincensini Laetitia L Reeg Nele N Buisson Johanna J Blisnick Thierry T Huet Diego D Perrot Sylvie S Santi-Rocca Julien J Duchateau Magalie M Hourdel Véronique V Rousselle Jean-Claude JC Cayet Nadège N Namane Abdelkader A Chamot-Rooke Julia J Bastin Philippe P

Molecular & cellular proteomics : MCP 20140416 7

Cilia and flagella are complex organelles made of hundreds of proteins of highly variable structures and functions. Here we report the purification of intact flagella from the procyclic stage of Trypanosoma brucei using mechanical shearing. Structural preservation was confirmed by transmission electron microscopy that showed that flagella still contained typical elements such as the membrane, the axoneme, the paraflagellar rod, and the intraflagellar transport particles. It also revealed that fl ...[more]

PMID: 24741115

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