Plasmodium falciparum schizont phosphoproteome
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos, instrument model
ORGANISM(S): Plasmodium Falciparum (isolate 3d7)
SUBMITTER: 
Mark Collins
PROVIDER: PXD000070 | Pride | 2014-04-24
REPOSITORIES: Pride
Publications
Confident and sensitive phosphoproteomics using combinations of collision induced dissociation and electron transfer dissociation.
Collins Mark O MO Wright James C JC Jones Matthew M Rayner Julian C JC Choudhary Jyoti S JS
Journal of proteomics 20140321
We present a workflow using an ETD-optimised version of Mascot Percolator and a modified version of SLoMo (turbo-SLoMo) for analysis of phosphoproteomic data. We have benchmarked this against several database searching algorithms and phosphorylation site localisation tools and show that it offers highly sensitive and confident phosphopeptide identification and site assignment with PSM-level statistics, enabling rigorous comparison of data acquisition methods. We analysed the Plasmodium falciparu ...[more]
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