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Formalin-fixed Human Ovarian Cancer Tryptic Peptide LC-MS/MS

ABSTRACT: LC-MS/MS (LTQ-Orbitrap, CID) data sets of 31 human ovarian cancer samples (FFPE). Sample material obtained by laser microdissection of cores from tissue microarray. Preparation matching MALDI imaging protocol (as described in Gustafsson, J Proteomics 2012).

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Stephan Meding

PROVIDER: PXD000079 | Pride | 2013-02-28

REPOSITORIES: Pride

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Tryptic peptide reference data sets for MALDI imaging mass spectrometry on formalin-fixed ovarian cancer tissues.

Meding Stephan S Martin Karina K Gustafsson Ove J R OJ Eddes James S JS Hack Sandra S Oehler Martin K MK Hoffmann Peter P

Journal of proteome research 20121213 1

MALDI imaging mass spectrometry is a powerful tool for morphology-based proteomic tissue analysis. However, peptide identification is still a major challenge due to low S/N ratios, low mass accuracy and difficulties in correlating observed m/z species with peptide identities. To address this, we have analyzed tryptic digests of formalin-fixed paraffin-embedded tissue microarray cores, from 31 ovarian cancer patients, by LC-MS/MS. The sample preparation closely resembled the MALDI imaging workflo ...[more]

PMID: 23214983

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