Proteomics

Dataset Information

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Streptococcus uberis extracellular proteome


ABSTRACT: S. uberis proteins from sub-cellular fractions (both cell wall-attached and secreted) were separated with SDS-PAGE, trypsine digested and identified with MS/MS analysis. Extracellular proteins were clustered into functional group based on in silico analysis. Concerning the database search: Mascot 2.2.0 was used with the following parameters: significance threshold p<0.05, Mascot MS/MS ion search : database NCBI, allow up to 1 miss cleavage, fixed modification - carbamidomethyl(C), variable modification- oxidation, peptide tol. +/- 1.5 Da, MS/MS tol. +/- 0.5 Da, monoisotopic.

INSTRUMENT(S): Bruker Daltonics esquire series, instrument model

ORGANISM(S): Streptococcus

SUBMITTER: Anita Jaglarz  

PROVIDER: PXD000142 | Pride | 2013-02-13

REPOSITORIES: Pride

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