Identifying RasGAP SH2 binding partners in Drosophila using mass spectrometry

ABSTRACT: Drosophila S2 cells were transfected with RasGAP(WT), RasGAP(SH2*32*) and GFP control. 4 mg of S2 cell lysate was used to pulldown LAP and GFP tagged constructs using GFP-Trap. The pulled down fraction was loaded on SDS-PAGE gels and coomassie stained before several bands (a-u) from two independent but parallel experiments were excised and subjected to MS analysis.

INSTRUMENT(S):

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Behzad Rowshanravan  

LAB HEAD: Behzad Rowshanravan

PROVIDER: PXD000176 | Pride | 2014-05-20

REPOSITORIES: Pride