Proteomics

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Quantitative Proteomics of miR-148a in gastric cancer cells


ABSTRACT: A quantitative proteomics combined with stable isotope labeling was applied to identify the global profile of miR-148a-regulated downstream proteins in AGS cancer cells. For proteomic analysis, cells were treated with miR-148a mimic (Pre-miR-148a) or miR-148a negative control (miR-CTL) and the downstream protein expression level (Pre-miR-148a/miR-CTL) were quantified using iTRAQ approach. Bioinformatics pipeline: The peak list in the resultant MS/MS spectra were generated by Mascot Distiller v2.1.1.0  and searched using Mascot v2.2 against the International Protein Index (IPI) human database (v. 3.64, 84032 sequences). The Mascot search parameters were +-0.1 Da for MS tolerance, +-0.1 Da for MS/MS mass tolerance, allowances for two missed cleavages, and variable modifications of deamidation (NQ), oxidation (M), iTRAQ (N terminal), iTRAQ (K), and MMTS (C). Protein quantitation were calculated using the Multi-Q software v1.6.5.4 with a dynamic range filter of ion count > 30.

INSTRUMENT(S): instrument model, MALDI Synapt G2 HDMS

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stomach, Epithelial Cell, Cell Culture

SUBMITTER: Chia-Wei Hu  

PROVIDER: PXD000190 | Pride | 2013-07-24

REPOSITORIES: Pride

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Publications

Quantitative proteomics reveals diverse roles of miR-148a from gastric cancer progression to neurological development.

Hu Chia-Wei CW   Tseng Chien-Wei CW   Chien Chih-Wei CW   Huang Hsuan-Cheng HC   Ku Wei-Chi WC   Lee Shyh-Jye SJ   Chen Yu-Ju YJ   Juan Hsueh-Fen HF  

Journal of proteome research 20130805 9


MicroRNAs (miRNAs) are noncoding RNAs that control gene expression either by degradation of mRNAs or inhibition of protein translation. miR-148a has been reported to have the impacts on tumor progression. Here, a quantitative proteomics combined with stable isotope labeling was applied to identify the global profile of miR-148a-regulated downstream proteins. The data have been deposited to the ProteomeXchange with identifier PXD000190. A total of 2938 proteins were quantified, and 55 proteins we  ...[more]

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