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Nipped in the bud: a microbial effector destabilizes MADS-domain transcription factors

ABSTRACT: Eluates from pull down experiments were separated on an SDS gel, and bands isolated for in gel trypsin digestion. Digested samples were analysed by LCMSMS on an Orbitrap. Peaklists were generated using MaxQuant 1.3.0.5 and database searches performed using Mascot Server 2.4. Data from all gel slices from one sample were merged in one Mascot search, and the results were imported into Scaffold 4.0.4.

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Gerhard Saalbach

PROVIDER: PXD000223 | Pride | 2014-05-21

REPOSITORIES: Pride

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Phytoplasma effector SAP54 hijacks plant reproduction by degrading MADS-box proteins and promotes insect colonization in a RAD23-dependent manner.

MacLean Allyson M AM Orlovskis Zigmunds Z Kowitwanich Krissana K Zdziarska Anna M AM Angenent Gerco C GC Immink Richard G H RG Hogenhout Saskia A SA

PLoS biology 20140408 4

Pathogens that rely upon multiple hosts to complete their life cycles often modify behavior and development of these hosts to coerce them into improving pathogen fitness. However, few studies describe mechanisms underlying host coercion. In this study, we elucidate the mechanism by which an insect-transmitted pathogen of plants alters floral development to convert flowers into vegetative tissues. We find that phytoplasma produce a novel effector protein (SAP54) that interacts with members of the ...[more]

PMID: 24714165

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