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Analysis of the peptidome of the moss Physcomitrella patens

ABSTRACT: We performed the analysis of native peptides pool of gametophores, protonema and protoplast cells of the moss. To reduce endogenous proteolytic activity we used an acid extraction buffer with a mixture of plant protease inhibitors. All the actions were performed on ice. After the proper sample preparation, the extracted peptides were identified with use of tandem mass spectrometry on the basis of protein database uniref100, taxon Physcomitrella patens.

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Physcomitrella Patens

TISSUE(S): Plant Cell

SUBMITTER: Dmitry Alexeev

LAB HEAD: Vadim Govorun

PROVIDER: PXD000227 | Pride | 2016-05-18

REPOSITORIES: Pride

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Specific pools of endogenous peptides are present in gametophore, protonema, and protoplast cells of the moss Physcomitrella patens.

Fesenko Igor A IA Arapidi Georgij P GP Skripnikov Alexander Yu AY Alexeev Dmitry G DG Kostryukova Elena S ES Manolov Alexander I AI Altukhov Ilya A IA Khazigaleeva Regina A RA Seredina Anna V AV Kovalchuk Sergey I SI Ziganshin Rustam H RH Zgoda Viktor G VG Novikova Svetlana E SE Semashko Tatiana A TA Slizhikova Darya K DK Ptushenko Vasilij V VV Gorbachev Alexey Y AY Govorun Vadim M VM Ivanov Vadim T VT

BMC plant biology 20150315

<h4>Background</h4>Protein degradation is a basic cell process that operates in general protein turnover or to produce bioactive peptides. However, very little is known about the qualitative and quantitative composition of a plant cell peptidome, the actual result of this degradation. In this study we comprehensively analyzed a plant cell peptidome and systematically analyzed the peptide generation process.<h4>Results</h4>We thoroughly analyzed native peptide pools of Physcomitrella patens moss ...[more]

PMID: 25848929

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