Mouse kidney mitochondria LC-MS/MS

ABSTRACT: ApoE KO mice were treated with Mas receptor agonist AVE 0991 fo 16 weeks. At the age of 6 months mice were killed, the kidneys were dissected and the mitochondria were isolated by differential centrifugation. Next, 2DE electrophoresis was conduced. Healthy control (C57BL/6J mice), apoE KO and apoE KO treated with AVE 0991 were compared. Gel pieces containing protein spots of interest were destained, reduced, alkylated, digested with modified trypsin and analysed by LC-MS/MS method.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Kidney

SUBMITTER: Maciej Suski  

LAB HEAD: Ryszard Korbut

PROVIDER: PXD000233 | Pride | 2016-05-09

REPOSITORIES: Pride