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Systems Biology Proteomic Analysis of Cerebral Cavernous Malformation

ABSTRACT: Cerebral cavernous malformations are vascular anomalies that can cause hemorrhagic stroke. Mutations in genes encoding Krit 1 (CCM1), OSM (CCM2), and PDCD10 (CCM3) proteins cause CCM. A loss in teh expression of any of these CCM proteins disrupts normal cerebral vessel development by disrupting the cytoskeleton and thereby inhibits endothelial tube ofrmation. Examination of cellular changes based on the loss of CCM gene expression may lead to the methods for early detection and prevention of CCM associated hemorrhagic stroke.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Kevin Knagge

PROVIDER: PXD000362 | Pride | 2014-06-09

REPOSITORIES: Pride

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Systems biology and proteomic analysis of cerebral cavernous malformation.

Edelmann Alexander R AR Schwartz-Baxter Sarah S Dibble Christopher F CF Byrd Warren C WC Carlson Jim J Saldarriaga Ivandario I Bencharit Sompop S

Expert review of proteomics 20140331 3

<h4>Unlabelled</h4>Cerebral cavernous malformations (CCM) are vascular anomalies caused by mutations in genes encoding KRIT1, OSM and PDCD10 proteins causing hemorrhagic stroke. We examine proteomic change of loss of CCM gene expression. Using human umbilical vein endothelial cells, label-free differential protein expression analysis with multidimensional liquid chromatography/tandem mass spectrometry was applied to three CCM protein knockdown cell lines and two control cell lines: ProteomeXchan ...[more]

PMID: 24684205

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