Dataset Information

MetaProSIP protein-SIP data - MetaProSIP: automated inference of stable isotope incorporation rates in proteins for functional metaproteomics

ABSTRACT: MetaProSIP offers an automated high-throughput solution for 13C or 15N protein-SIP experiments. Labeled peptides are identified based on their unlabeled isotopologues. It calculates incorporation of heavy isotopes (RIA), the labeling ratio (LR) between proteins as well as additional shape properties of the isotopic distribution. FeatureXML files have been created using OpenMS 1.10 using the FeatureFinderCentroided tool. Unlabeled peptides have been identified by OMSSA 2.1.9 (precursor mass tolerance 10 ppm, fragment mass tolerance 0.5 Da, FDR filtering at 2%) and mapped to the feature data using the IDMapper tool.

INSTRUMENT(S):

ORGANISM(S): Uncultured Bacterium

SUBMITTER: Timo Sachsenberg