Proteomics

Dataset Information

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Subcellular proteomic survey of Salmonella

ABSTRACT: The project performed a subcellular proteomic analysis of Salmonella enterica subsp. enterica serovar Typhimurium str. 14028 grown under standard laboratory and phagosome-mimicking conditions in vitro. Analysis of proteins from cytoplasmic, inner membrane, periplasmic, and outer membrane fractions yielded coverage of 25% of the theoretical proteome. Confident subcellular location could be assigned to over 1000 proteins, with good agreement between experimentally observed location and predicted/known protein properties. Comparison of protein location under the different environmental conditions provided insight into dynamic protein localization and possible moonlighting (multiple function) activities.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Salmonella Typhimurium (strain 14028s / Sgsc 2262)

SUBMITTER: Matthew Monroe  

PROVIDER: PXD000388 | Pride | 2013-10-07

REPOSITORIES: Pride

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Publications

A Comprehensive Subcellular Proteomic Survey of Salmonella Grown under Phagosome-Mimicking versus Standard Laboratory Conditions.

Brown Roslyn N RN   Sanford James A JA   Park Jea H JH   Deatherage Brooke L BL   Champion Boyd L BL   Smith Richard D RD   Heffron Fred F   Adkins Joshua N JN  

International journal of proteomics 20120725

Towards developing a systems-level pathobiological understanding of Salmonella enterica, we performed a subcellular proteomic analysis of this pathogen grown under standard laboratory and phagosome-mimicking conditions in vitro. Analysis of proteins from cytoplasmic, inner membrane, periplasmic, and outer membrane fractions yielded coverage of 25% of the theoretical proteome. Confident subcellular location could be assigned to over 1000 proteins, with good agreement between experimentally observ  ...[more]