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Time course response of macrophages to Salmonella infection

ABSTRACT: To investigate the extent to which macrophages respond to Salmonella infection, researchers infected RAW 264.7 macrophages with Salmonella enterica serotype Typhimurium and analyzed macrophage proteins at various time points following infection by using a global proteomic approach.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Salmonella Typhimurium (strain 14028s / Sgsc 2262)

SUBMITTER: Matthew Monroe

PROVIDER: PXD000389 | Pride | 2013-10-07

REPOSITORIES: Pride

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Proteomic investigation of the time course responses of RAW 264.7 macrophages to infection with Salmonella enterica.

Shi Liang L Chowdhury Saiful M SM Smallwood Heather S HS Yoon Hyunjin H Mottaz-Brewer Heather M HM Norbeck Angela D AD McDermott Jason E JE Clauss Therese R W TR Heffron Fred F Smith Richard D RD Adkins Joshua N JN

Infection and immunity 20090615 8

To investigate the extent to which macrophages respond to Salmonella infection, we infected RAW 264.7 macrophages with Salmonella enterica serotype Typhimurium and analyzed macrophage proteins at various time points following infection by using a global proteomic approach. A total of 1,006 macrophage and 115 Salmonella proteins were identified with high confidence. Most of the Salmonella proteins were observed in the late stage of the infection time course, which is consistent with the fact that ...[more]

PMID: 19528222

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