Proteomics

Dataset Information

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Ndd1 phosphorylation pattern upon DNA damage


ABSTRACT: The transcriptional activator NDD1 was purified from wt and rad53 or mec1 mutant treated with hydroxyurea or methyl-methanesulfonate via HTB-tag , digested directly from the streptavidin beads, and then subjected to LC-MSMS analysis. Beads were alternatively digested with trypsin and chymotrypsin for better sequence coverage.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Dorothea Anrather  

PROVIDER: PXD000405 | Pride | 2013-12-19

REPOSITORIES: Pride

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Publications

Genotoxic stress prevents Ndd1-dependent transcriptional activation of G2/M-specific genes in Saccharomyces cerevisiae.

Yelamanchi Syam Kumar SK   Veis Jiri J   Anrather Dorothea D   Klug Helene H   Ammerer Gustav G  

Molecular and cellular biology 20131209 4


Downregulation of specific transcripts is one of the mechanisms utilized by eukaryotic checkpoint systems to prevent cell cycle progression. Here we identified and explored such a mechanism in the yeast Saccharomyces cerevisiae. It involves the Mec1-Rad53 kinase cascade, which attenuates G(2)/M-specific gene transcription upon genotoxic stress. This inhibition is achieved via multiple Rad53-dependent inhibitory phosphorylations on the transcriptional activator Ndd1 that prevent its chromatin rec  ...[more]

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