Quantitative proteomics of cryptococcal meningitis brain tissues

ABSTRACT: Quantitative proteomics of cryptococcal meningitis brain tissues and control brain tissues was carried out to study the host factors that are activated and differentially regulated in response to infection. Proteins isolated from infected brain tissues were subjected to trypsin digestion and then labeled using 4-plex iTRAQ labels. iTRAQ labeled peptides were subjected to fractionation by SCX. All the fractions were analyzed on the high resolution Orbitrap Velos mass spectrometer.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Akhilesh Pandey  

LAB HEAD: Akhilesh Pandey

PROVIDER: PXD000458 | Pride | 2015-07-22

REPOSITORIES: Pride