Proteomics

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Comparison of saliva proteins signature of three aphid species


ABSTRACT: In this study we used a multidisciplinary proteomics approach combining LC-ESI-MS/MS and 2D-DIGE/MALDI-TOF/MS to compare the salivary proteins from three aphid species including Acyrthosiphon pisum, Megoura viciae, and Myzus persicae. Comparative analyses revealed variability among aphid salivary proteomes. Among the proteins that varied 22% were related to DNA-binding, 19% were related to GTP-binding, and 19% had oxidoreductase activity.

INSTRUMENT(S): instrument model

ORGANISM(S): Acyrthosiphon Pisum (pea Aphid) Myzus Persicae Megoura Viciae (vetch Aphid)

TISSUE(S): Saliva

SUBMITTER: Frederic FRANCIS  

LAB HEAD: Frederic FRANCIS

PROVIDER: PXD000476 | Pride | 2017-08-17

REPOSITORIES: Pride

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Publications

Comparative analyses of salivary proteins from three aphid species.

Vandermoten S S   Harmel N N   Mazzucchelli G G   De Pauw E E   Haubruge E E   Francis F F  

Insect molecular biology 20131101 1


Saliva is a critical biochemical interface between aphids and their host plants; however, the biochemical nature and physiological functions of aphid saliva proteins are not fully elucidated. In this study we used a multidisciplinary proteomics approach combining liquid chromatography-electrospray ionization tandem mass spectrometry and two-dimensional differential in-gel electrophoresis/matrix-assisted laser desorption/ionization time-of-flight/mass spectrometry to compare the salivary proteins  ...[more]

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